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total rap1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology total rap1
    Total Rap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 101 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+rap1/Rap+1A+Antibody/ppr0356476-81-70-76
    Average 93 stars, based on 101 article reviews
    total rap1 - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: GGTase-I deficiency reduces tumor formation and improves survival in mice with K-RAS-induced lung cancer
    Article Snippet: The proteins were transferred to nitrocellulose membranes and incubated with antibodies recognizing nonprenylated RAP1A (catalog no. sc-1482), total RAP1 (catalog no. sc-65), RHOA (catalog no. sc-418), p21 CIP1 (catalog no. sc-6246), cyclin D1 (catalog no. sc-450), and the HA tag (catalog no. sc-7392) (Santa Cruz Biotechnology Inc.), phosphorylated ERK1/2 (catalog no. 9106), phosphorylated AKT Ser473 (catalog no. 9271), total AKT (catalog no. 9272), total ERK1/2 (catalog no. 9102), CDC42 (catalog no. 2462) (Cell Signaling Technology), and the Myc tag (catalog no. R950-25; Invitrogen).

    Article Title: JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling.
    Article Snippet: Rabbit polyclonal anti-Rap1 (Santa Cruz Biotechnology) and anti-rabbit HRPconjugated (Cells Signaling Technology) antibodies were used to reveal Rap1-GTP and total Rap1.

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Efficient knockdown was usually attained within 72 hrs and confirmed regularly by either western blot using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, SC-65) or reverse-transcriptase PCR using isoform-specific primers.

    Article Title: The Small GTPase Rap1 Is a Novel Regulator of RPE Cell Barrier Function
    Article Snippet: Knockdown was confirmed regularly by Western blot analysis of cell lysates using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Article Title: Inhibitors of Protein Geranylgeranyltransferase I and Rab Geranylgeranyltransferase Identified from a Library of Allenoate-derived Compounds
    Article Snippet: Whole cell lysates of NIH3T3 cells were electrophoresed on a 12% SDS-polyacrylamide gel, transferred to nitrocellulose membranes, and immunoblotted with antibody against the unprenylated form of Rap1 (goat, catalog no. sc-1482, Santa Cruz Biotechnology, Inc.), total Rap1 (catalog no. sc-65, Santa Cruz Biotechnology, Inc.) or actin (catalog no. A4700, Sigma).

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Briefly, 293A cells were transfected with PacI-digested pAd-CMV-Dest vector containing the desired miRNA cassette, using Lipofectamine 2000 (Invitrogen, 11668-019).

    Blocking Assay:

    Article Title: GGTase-I deficiency reduces tumor formation and improves survival in mice with K-RAS-induced lung cancer
    Article Snippet: The proteins were transferred to nitrocellulose membranes and incubated with antibodies recognizing nonprenylated RAP1A (catalog no. sc-1482), total RAP1 (catalog no. sc-65), RHOA (catalog no. sc-418), p21 CIP1 (catalog no. sc-6246), cyclin D1 (catalog no. sc-450), and the HA tag (catalog no. sc-7392) (Santa Cruz Biotechnology Inc.), phosphorylated ERK1/2 (catalog no. 9106), phosphorylated AKT Ser473 (catalog no. 9271), total AKT (catalog no. 9272), total ERK1/2 (catalog no. 9102), CDC42 (catalog no. 2462) (Cell Signaling Technology), and the Myc tag (catalog no. R950-25; Invitrogen).

    Article Title: JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling.
    Article Snippet: Rabbit polyclonal anti-Rap1 (Santa Cruz Biotechnology) and anti-rabbit HRPconjugated (Cells Signaling Technology) antibodies were used to reveal Rap1-GTP and total Rap1.

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Efficient knockdown was usually attained within 72 hrs and confirmed regularly by either western blot using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, SC-65) or reverse-transcriptase PCR using isoform-specific primers.

    Article Title: The Small GTPase Rap1 Is a Novel Regulator of RPE Cell Barrier Function
    Article Snippet: Knockdown was confirmed regularly by Western blot analysis of cell lysates using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Article Title: Inhibitors of Protein Geranylgeranyltransferase I and Rab Geranylgeranyltransferase Identified from a Library of Allenoate-derived Compounds
    Article Snippet: Whole cell lysates of NIH3T3 cells were electrophoresed on a 12% SDS-polyacrylamide gel, transferred to nitrocellulose membranes, and immunoblotted with antibody against the unprenylated form of Rap1 (goat, catalog no. sc-1482, Santa Cruz Biotechnology, Inc.), total Rap1 (catalog no. sc-65, Santa Cruz Biotechnology, Inc.) or actin (catalog no. A4700, Sigma).

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Briefly, 293A cells were transfected with PacI-digested pAd-CMV-Dest vector containing the desired miRNA cassette, using Lipofectamine 2000 (Invitrogen, 11668-019).

    Western Blot:

    Article Title: GGTase-I deficiency reduces tumor formation and improves survival in mice with K-RAS-induced lung cancer
    Article Snippet: The proteins were transferred to nitrocellulose membranes and incubated with antibodies recognizing nonprenylated RAP1A (catalog no. sc-1482), total RAP1 (catalog no. sc-65), RHOA (catalog no. sc-418), p21 CIP1 (catalog no. sc-6246), cyclin D1 (catalog no. sc-450), and the HA tag (catalog no. sc-7392) (Santa Cruz Biotechnology Inc.), phosphorylated ERK1/2 (catalog no. 9106), phosphorylated AKT Ser473 (catalog no. 9271), total AKT (catalog no. 9272), total ERK1/2 (catalog no. 9102), CDC42 (catalog no. 2462) (Cell Signaling Technology), and the Myc tag (catalog no. R950-25; Invitrogen).

    Article Title: JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling.
    Article Snippet: Rabbit polyclonal anti-Rap1 (Santa Cruz Biotechnology) and anti-rabbit HRPconjugated (Cells Signaling Technology) antibodies were used to reveal Rap1-GTP and total Rap1.

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Efficient knockdown was usually attained within 72 hrs and confirmed regularly by either western blot using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, SC-65) or reverse-transcriptase PCR using isoform-specific primers.

    Article Title: The Small GTPase Rap1 Is a Novel Regulator of RPE Cell Barrier Function
    Article Snippet: Knockdown was confirmed regularly by Western blot analysis of cell lysates using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Article Title: Inhibitors of Protein Geranylgeranyltransferase I and Rab Geranylgeranyltransferase Identified from a Library of Allenoate-derived Compounds
    Article Snippet: Whole cell lysates of NIH3T3 cells were electrophoresed on a 12% SDS-polyacrylamide gel, transferred to nitrocellulose membranes, and immunoblotted with antibody against the unprenylated form of Rap1 (goat, catalog no. sc-1482, Santa Cruz Biotechnology, Inc.), total Rap1 (catalog no. sc-65, Santa Cruz Biotechnology, Inc.) or actin (catalog no. A4700, Sigma).

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Briefly, 293A cells were transfected with PacI-digested pAd-CMV-Dest vector containing the desired miRNA cassette, using Lipofectamine 2000 (Invitrogen, 11668-019).

    Polymerase Chain Reaction:

    Article Title: GGTase-I deficiency reduces tumor formation and improves survival in mice with K-RAS-induced lung cancer
    Article Snippet: The proteins were transferred to nitrocellulose membranes and incubated with antibodies recognizing nonprenylated RAP1A (catalog no. sc-1482), total RAP1 (catalog no. sc-65), RHOA (catalog no. sc-418), p21 CIP1 (catalog no. sc-6246), cyclin D1 (catalog no. sc-450), and the HA tag (catalog no. sc-7392) (Santa Cruz Biotechnology Inc.), phosphorylated ERK1/2 (catalog no. 9106), phosphorylated AKT Ser473 (catalog no. 9271), total AKT (catalog no. 9272), total ERK1/2 (catalog no. 9102), CDC42 (catalog no. 2462) (Cell Signaling Technology), and the Myc tag (catalog no. R950-25; Invitrogen).

    Article Title: JAM-A promotes neutrophil chemotaxis by controlling integrin internalization and recycling.
    Article Snippet: Rabbit polyclonal anti-Rap1 (Santa Cruz Biotechnology) and anti-rabbit HRPconjugated (Cells Signaling Technology) antibodies were used to reveal Rap1-GTP and total Rap1.

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Efficient knockdown was usually attained within 72 hrs and confirmed regularly by either western blot using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, SC-65) or reverse-transcriptase PCR using isoform-specific primers.

    Article Title: The Small GTPase Rap1 Is a Novel Regulator of RPE Cell Barrier Function
    Article Snippet: Knockdown was confirmed regularly by Western blot analysis of cell lysates using a polyclonal antibody that recognizes total Rap1 (Santa Cruz Biotechnology, Santa Cruz, CA).

    Article Title: Inhibitors of Protein Geranylgeranyltransferase I and Rab Geranylgeranyltransferase Identified from a Library of Allenoate-derived Compounds
    Article Snippet: Whole cell lysates of NIH3T3 cells were electrophoresed on a 12% SDS-polyacrylamide gel, transferred to nitrocellulose membranes, and immunoblotted with antibody against the unprenylated form of Rap1 (goat, catalog no. sc-1482, Santa Cruz Biotechnology, Inc.), total Rap1 (catalog no. sc-65, Santa Cruz Biotechnology, Inc.) or actin (catalog no. A4700, Sigma).

    Article Title: Isoform-specific differences between Rap1A and Rap1B GTPases in the formation of endothelial cell junctions
    Article Snippet: Briefly, 293A cells were transfected with PacI-digested pAd-CMV-Dest vector containing the desired miRNA cassette, using Lipofectamine 2000 (Invitrogen, 11668-019).



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    FIGURE 6. Expression of FPPS and <t>Rap1</t> in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.
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    FIGURE 6. Expression of FPPS and <t>Rap1</t> in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.
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    FIGURE 6. Expression of FPPS and <t>Rap1</t> in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.
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    FIGURE 6. Expression of FPPS and <t>Rap1</t> in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.
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    Image Search Results


    FIGURE 6. Expression of FPPS and Rap1 in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Butyrophilin 3A/CD277-Dependent Activation of Human γδ T Cells: Accessory Cell Capacity of Distinct Leukocyte Populations.

    doi: 10.4049/jimmunol.1600913

    Figure Lengend Snippet: FIGURE 6. Expression of FPPS and Rap1 in monocytes and neutro- phils. Highly purified monocytes and neutrophils were incubated in me- dium alone or in the presence of 2, 10, or 50 mM of ZOL. After 3 h, the cells were washed with culture medium and incubated overnight in fresh medium. Cells were collected, washed with PBS, and lysed. Five micro- grams of cellular lysates were separated by SDS-PAGE. Proteins were transferred to nitrocellulose followed by immunoblot with anti-Rap1A/B mAb (clone 26B4) to detect total Rap1, with polyclonal Ab C-17 to detect unprenylated Rap1A (uRap1A), or anti-FPPS polyclonal Ab and appro- priate HRP-conjugated secondary Ab. Blots were stripped and GAPDH served as a loading control. Comparable results were obtained in a second independent experiment.

    Article Snippet: Western blots were separately analyzed using rabbit polyclonal Ab against FPPS (PA5-28228; Thermo Fisher Scientific), goat polyclonal Ab against unprenylated Rap 1A (C-17, sc-1482; Santa Cruz Biotechnology, Santa Cruz, CA) or rabbit mAb against total Rap1 (2399; Cell Signaling Technology, Danvers, MA) and processed as described previously (37) with corresponding HRP-coupled secondary Ab and chemiluminescence reagents from GE Healthcare (Freiburg, Germany).

    Techniques: Expressing, Incubation, SDS Page, Western Blot, Control